Two lasers of different wavelengths create two distinct images, a fluorescence image and a reflectance image. Both signals are scanned simultaneously and are used to create pseudo-colored images. The device’s software uses an algorithm to translate the acquired image information into colors that resemble H&E.
Images courtesy of Dr Javiera Pérez-Anker.
Basal cell carcinoma; imaged with the VivaScope 2500 (left) and after H&E staining (right)